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Low Dose Gamma Irradiation Potentiates Secondary Exposure to Gamma Rays or Protons in Thyroid Tissue Analogs
(jointly funded by NASA and DOE)
Lora
M. Green
lgreen@dominion.llumc.edu
Radiobiolgy Program, Loma Linda University, Loma Linda CA 92354
Website: http://www.llu.edu/llu/faculty/directory/faculty.html?id=lgreen
Why this Project:
Homeostatic processes may be responsible for the adaptive responses triggered by exposure to low doses of radiation.
Project Goals:
To characterize genes expressed after irradiation involved in:
- radiation-induced adaptive response (a potentially protective low dose phenomenon)
- bystander effects (effects in neighboring unirradiated cells)
Experimental Approach:
Using a reconstituted three-dimensional tissue model derived from rat thyroid (FRTL-5) tissue. Following irradiation using various exposure protocols, the reconstituted tissues will be characterized for the profile of genes that they express. Effects of irradiation on bystander cells will be addressed using a micro-collimated high-energy proton beam irradiator.
Expected Outcomes:
The project will determine if epithelial cells in a normal matrix have similar adaptive and bystander responses as fibroblasts or lymphocytes grown as single isolated cells. Such information is critical in extrapolating molecular information to cancer risks.
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