Setup for microbeam irradiation of tissue fragments. The human skin artificial tissue system is cultivated on a Millicell-CM culture insert, which utilizes a 28 µ m hydrophilic membrane. For irradiation, the insert is positioned in a custom-designed holder ( A ) and is held down by a fixture ( B ) to maintain stability. The insert holder is positioned on the microbeam stage, and the tissue is irradiated from below. The full microbeam cell dish assembly is represented in D .

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